ile-peptide In the intricate field of proteomics and peptide analysis, achieving high sensitivity, accuracy, and throughput is paramount. Waters has emerged as a key innovator with its iKey HT Peptide Separation Devices, a series of advanced chromatographic tools designed to meet these demanding requirements(PDF) Practical applications of integrated microfluidics for .... These devices are integral components of the ionKey/MS system, offering a streamlined and efficient approach to peptide and protein bioanalysis.
The iKey Separation Device itself represents a significant technological leap, eliminating the need for traditional fittings and simplifying the setup process for microflow Liquid Chromatography coupled to Mass Spectrometry (LC-MS). This miniaturization, particularly with the iKey HT separation device, leads to increased sensitivity and reduced cycle times, making it an invaluable asset for laboratories focused on detailed peptide analysisTheiKey™Separation Deviceis one of the enabling technologies behind the ionKey/MS™ System. The innovativeiKeyeliminates the need for traditional ....
Several variations of the iKey HT Peptide Separation Device are available, catering to specific analytical needs.iKey HT Peptide CSH C18 Separation Devi Among these are the iKey HT Peptide BEH C18 Separation Device and the iKey HT Peptide CSH C18 Separation Device. Both leverage advanced stationary phases, such as BEH C18 and CSH C18, known for their exceptional performance in separating complex peptide mixturesUniversity of Groningen Mass spectrometry-based methods .... These devices are typically characterized by specific pore sizes, such as 130 Å, and particle sizes, like 1.7 µm, which are crucial for optimizing chromatographic resolution and efficiency2019年2月8日—Chromatographicseparationwas achieved on a C18-bonded WatersiKeyHSS T3Separation Device(1.8 μm particles, 100. Å pore size, 150 μm × 100 .... The iKey HT Peptide BEH C18 Separation Device, for instance, is designed for robust performance with a 130 Å pore size and 1.7 µm particles, ensuring excellent peak symmetry and accurate quantitation. Similarly, the Waters iKey HT Peptide CSH C18 Separation Device offers a complementary stationary phase option for researchers exploring different separation mechanisms.
The "HT" in iKey HT signifies "High Throughput.Waters iKey HT Peptide BEH C18 Separation Device, 130Ã?, 1.7 ..." This is achieved through features like a 300 µm I.D. separation channel, which allows for faster sample processing compared to lower-flow systems, significantly increasing laboratory throughputBiomolecule Purification, Characterization, and Analyses. This enhanced speed, combined with the inherent sensitivity of microflow LC, makes the iKey HT separation device ideal for applications requiring rapid analysis of large sample sets, such as in drug discovery or clinical diagnostics. The iKey HT is a testament to Waters' commitment to developing innovative separation devicesWaters iKey HT Peptide CSH C18 Separation Device, 130Å, 1.7 µm, 300 µm X 50 mm, 1/pk ; 케미스트리, C18 ; 분리 모드, 역상 ; 입자 기질, Hybrid ; pH Range Min, 1 pH ....
Beyond the BEH and CSH chemistries, other iKey Peptide Separation Devices exist, including the ionKey HSS T3 Separation DeviceAnalyticalseparationwas performed on a Waters BEH 130 C18iKey™Separation Device. (100 mm x 150 μm I.D., 1.7 μm). Gradient conditions were as follows: 0.0 .... This variation, often featuring a 100 Å pore size and 1iKey Peptide Separation Devices, 1/pk. Dimension. P/N. Particle Size: 1.7 µm. BEH C18, 130Е. 150 µm × 50 mm. 186006764. 150 µm × 50 mm (PCA). 186007557. 150 µm ....8 µm particles, provides an alternative for specific analytical challenges, demonstrating the versatility of the iKey platform. The iKey Separation Device is manufactured to exacting specifications, ensuring consistent and reliable performance across numerous runs.
The iKey platform is not just about the device itself; it's about the integrated ionKey/MS systemThe 300 µm I.D.iKey HT Separation Deviceallows higher sample throughput with short cycle times; Simplified microflow LC-MS user experience; UPLC separations .... This synergy allows for a simplified microflow LC-MS user experience, enabling UPLC separations with unparalleled ease. The innovative nature of the iKey eliminates the need for traditional, often cumbersome, fittings, streamlining the entire analytical workflow. This focus on user-friendliness and efficiency is a hallmark of Waters' approach to chromatographic solutions.
Applications leveraging these iKey HT Peptide Separation Devices are diverse and impactful. They are employed in the analysis of complex biological samples, including peptides from serum and other biological fluids, for biomarker discovery and validation. The ability to achieve high-resolution separation of peptides is fundamental to understanding protein function and dysfunction in health and disease作者:F Klont·2018—separationwas achieved on a C18-bonded WatersiKeyHSS T3Separation Device(1.8 μm particles, 100 Å pore size, 150 μm × 100 mm; Cat. No .... Researchers utilize these devices for applications ranging from protein relative quantification to the detection of specific biomarkers. The iKey is a versatile tool for separation in various analytical contexts.
In summary, the iKey HT Peptide Separation Devices from Waters offer a powerful and integrated solution for advanced peptide and protein analysisWaters iKey HT Peptide BEH C18 분리 장치는 ionKey/MS 시스템용으로 설계된 혁신적인 마이크로플로우 LC 컬럼입니다. 피팅이 필요 없으며 사용이 간편하고, .... Their innovative design, coupled with high-performance stationary phases and compatibility with the ionKey/MS system, enables researchers to achieve higher sensitivity, improved accuracy, and increased throughput in their demanding proteomic studies. The iKey separation device is a critical component for modern mass spectrometry-based workflows.
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