inf7 peptide INF7 peptide consisting of cysteine on its C-terminus - injectable-peptide fusogenic peptides undergo a pHdependent conformational change The Multifaceted INF7 Peptide: A Key Player in Cellular Delivery and Membrane Fusion

inhalation-of-peptide-loaded-nanoparticles-improves-heart-failure The INF7 peptide has emerged as a significant molecule in biomolecular research, particularly for its remarkable ability to facilitate cellular uptake and manipulate cellular membranes. Derived from the N-terminal domain of the HA2 protein of the influenza virus, this peptide is characterized by its glutamine-enriched sequence and remarkable pH sensitivity. Its utility spans various applications, from enhancing gene expression to enabling targeted delivery of therapeutic agents.

At its core, the INF7 peptide functions as a fusogenic peptide. This property stems from its origin as a segment of the influenza virus hemagglutinin protein, which is crucial for viral entry into host cells. Upon encountering the acidic environment of endosomes, the INF7 peptide undergoes a pH-dependent conformational change, primarily forming an alpha-helical structure. This transformation allows it to disrupt the stability of endosomal membranes, a process vital for the release of encapsulated cargo into the cell's cytoplasmTargeted Lysosome Disruptive Elements for Improvement .... Studies have shown that INF7 is particularly promising in terms of cholesterol dependence and disruption kinetics, making it a valuable tool for manipulating cellular processes.

One of the most extensively researched applications of the INF7 peptide involves its use in enhancing intracellular delivery.作者：S Tarvirdipour·2021·被引用次数：29—Wild-type HA2(1–23) peptide anda glutamic acid-enriched analogue (INF7) from influenza virus hemagglutinin are the oldest and best studied fusogenic peptides ... By co-encapsulating the INF7 peptide with other molecules, researchers can significantly improve their cellular uptake. For instance, the INF7 peptide has been demonstrated to enhance the endosomal escape of various payloads, including proteins and nucleic acids.Protein HA2 domain derivative polypeptide, INF7,185462-59-3, polypeptide synthesis, polypeptide customization ; Grade. Pharmaceutical Grade ; Product Keywords. This capability is crucial for the effective delivery of gene therapy vectors, such as DNA or RNA, into target cells.作者：BR Liu·2013·被引用次数：42—INF7 peptide, a glutamic acid-enriched influenza virus hemagglutinin-2 (HA2) analogue, has been shown to be a particularly potent fusion peptide [59], as was ... The INF7 peptide can act as a spacer or linker, making other molecules, like the TAT peptide, more accessible for interaction with the cell surface, thereby facilitating cellular entry.

Furthermore, modifications to the INF7 peptide have yielded even more potent derivatives.作者：NM Moore·2008·被引用次数：89—Coupling oneINF7 peptideto PEG-DNA binding peptide (DBP) vehicles increased the transfection efficiency ten-fold and showed greater transfection efficiency ... The stearylated INF7 peptide, for example, has shown a remarkable ability to enhance endosomal escape and gene expressionA GalNAc-conjugated fusion peptidedesigned to enhance liver-targeted intracellular deliverythrough membrane fusion activity.. Research has indicated that a stearylated INF7 peptide (str-INF7) can be a useful device for enhancing the endosomal escape of even PEGylated liposomes. In one study, R8-MEND modified with stearylated INF7 peptide resulted in gene expression levels significantly higher than unmodified versions or free INF7...peptide INF7 (red squiggles) is endocytosed. The acidic pH of the late endosome activates the INF7 to permeabilize the liposomal and endosomal membranes and .... This demonstrates the potential of chemical modifications to amplify the peptide's inherent capabilitiesIntracellular Delivery of Nanoparticles and DNAs by IR9 Cell ....

The INF7 peptide also plays a role in targeted delivery systems. For instance, GalNAc-INF7 is a fusion peptide designed to enhance liver-targeted intracellular delivery through membrane fusion activity. This specific conjugation leverages the known affinity of N-acetylgalactosamine (GalNAc) for liver cells, directing the therapeutic payload precisely where it's needed作者：SMW van Rossenberg·2002·被引用次数：55—INF7, the most promising peptidein terms of cholesterol dependence and disruption ki- netics, was equipped with a homing device for the asialoglyco- protein ....

Beyond its direct role in membrane fusion, the INF7 peptide has been integrated into more complex delivery systems. The INF7-A5K-TAT peptide, for example, combines the fusogenic properties of INF7 with the cell-penetrating capabilities of TAT and the CRISPR RNP binding ability of A5KIntracellular Delivery of Nanoparticles and DNAs by IR9 Cell .... This tripartite peptide can non-covalently bind to CRISPR ribonucleoproteins and efficiently deliver them to various immune cells, such as primary human T cells, B cells, and NK cells, enabling precise gene editing2026年1月25日—Description：INF7是HA2 蛋白N 端结构域的衍生物多肽，是由24个氨基酸组成的多肽，可用于增强复合物或脂质体封装蛋白的内体逃逸。. The Peptide A5K (INF7-A5K-TAT) is an RNP delivery peptide that effectively edits T cells without substantial impact on their viability.

The inherent properties of the INF7 peptide also extend to its physical characteristics. At physiological pH (7.4), the INF7 peptide displays tryptophan emission and LD features indicative of a surface-oriented peptide, suggesting that its N-terminal glutamic acid residues play a role in its interaction with membranes. The INF7 peptide is a 24-amino acid polypeptide that can be used to enhance the intracellular transport of complexes or liposome-encapsulated proteins.Co-Encapsulating the Fusogenic Peptide INF7 and ...

In summary, the INF7 peptide is a versatile and powerful tool in the field of biomolecular research and drug delivery. Its pH-dependent conformational change, its ability to facilitate cellular uptake of a variety of molecular cargo, and its amenability to chemical modifications like stearylated INF7 peptide and conjugations like GalNAc-INF7 make it a cornerstone for developing advanced therapeutic strategies. From enhancing gene expression to enabling targeted gene editing, the Protein HA2 domain derivative polypeptide, INF7 continues to be a subject of intense study and innovation.INF7 | Polypeptide